CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.


CRISPR Formats

SygRNA® Synthetic gRNA

SygRNA® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9. When ordering sgRNA or crRNA:tracrRNA, be sure to select the species that matches your Cas9 source.

View our protocols to learn more about SygRNA® synthetic CRISPR.

Sanger QuickPick™ Knockout gRNA Lentivirus Manufacturing Timelines

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CRISPR gRNA Lentivirus Manufacturing Timelines

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Cas9 Products

Click on the product number to order and see additional product details

Cas9 Proteins

Cas9 Plasmids and Lentivirus

SAM CRISPR Activator Cas9 Products

CRISPR Inhibition Cas9 Products & Controls

Vectors

Mammalian System Vectors

Lentiviral Vectors

Plant Vectors

CRISPR Controls

Click on the product number to order and see additional product details

Volume Price Discounts

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol

Plasmid DNA: provided in 1xTris-EDTA

Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

For all custom inquiries or additional information Contact Us.

Vector Maps and Sequences

Click on the images below for a larger view.

p01 Vector

Download Fully Annotated p01 Map
Download p01 FASTA Sequence

p02 Vector

Download Fully Annotated p02 Map
Download p02 FASTA Sequence

p03 Vector

Download Fully Annotated p03 Map
Download p03 FASTA Sequence

p04 Vector

Download Fully Annotated p04 Map
Download p04 FASTA Sequence

p05 Vector

Download Fully Annotated p05 Map
Download p05 FASTA Sequence

p10 Vector

Download Fully Annotated p10 Map
Download p10 FASTA Sequence

p50 Vector

Download Fully Annotated p50 Map
Download p50 FASTA Sequence

p51 Vector

Download Fully Annotated p51 Map
Download p51 FASTA Sequence

p52 Vector

Download Fully Annotated p52 Map
Download p52 FASTA Sequence

p53 Vector

Download Fully Annotated p53 Map
Download p53 FASTA Sequence

p54 Vector

Download Fully Annotated p54 Map
Download p54 FASTA Sequence

p55 Vector

Download Fully Annotated p55 Map
Download p5 FASTA Sequence

p56 Vector

Download Fully Annotated p56 Map
Download p56 FASTA Sequence

p57 Vector

Download Fully Annotated p57 Map
Download p57 FASTA Sequence

p58 Vector

Download Fully Annotated p58 Map
Download p58 FASTA Sequence

p59 Vector

Download Fully Annotated p59 Map
Download p59 FASTA Sequence

p60 Vector

Download Fully Annotated p60 Map
Download p60 FASTA Sequence

p61 Vector

Download Fully Annotated p61 Map
Download p61 FASTA Sequence

p62 Vector

Download Fully Annotated p62 Map
Download p62 FASTA Sequence

p63 Vector

Download Fully Annotated p63 Map
Download p01 FASTA Sequence

LV01 Vector

Download Fully Annotated LV01 Map
Download LV01 FASTA Sequence

LV02 Vector

Download Fully Annotated LV02 Map
Download LV02 FASTA Sequence

LV03 Vector

Download Fully Annotated LV03 Map
Download LV03 FASTA Sequence

LV04 Vector

Download Fully Annotated LV04 Map
Download LV04 FASTA Sequence

LV05 Vector

Download Fully Annotated LV05 Map
Download LV05 FASTA Sequence

LV06 Vector

Download Fully Annotated LV06 Map
Download LV06 FASTA Sequence

LV07 Vector

Download Fully Annotated LV07 Map
Download LV07 FASTA Sequence

LV17 Vector

KRAB-dCas9 Vector

Product Guarantee

We are so confident in the performance of our SygRNA® products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.

To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA® gRNA. To receive your replacement, simply email oligotechserv@milliporesigma.com and include sample data from a representative experiment (T7E1, TIDE, or NGS)