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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
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Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material
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Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
Formaldehyde solution, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Supelco
2-Mercaptoethanol, derivatization grade (HPLC), LiChropur, ≥99.0% (GC)
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Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
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Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Acetone, United States Pharmacopeia (USP) Reference Standard
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Dimethylformamide, Pharmaceutical Secondary Standard; Certified Reference Material
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Chloroform, suitable for HPLC, ≥99.8%, amylene stabilized
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Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Methanol, suitable for HPLC, gradient grade, ≥99.9%
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Chloroform, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 0.5-1.0% ethanol as stabilizer
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N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
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Methanol, HPLC Plus, ≥99.9%
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Acetone, suitable for HPLC, ≥99.9%
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Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
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1-Octanol, suitable for HPLC, ≥99%
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Diethyl ether, suitable for HPLC, ≥99.9%, inhibitor-free
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Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Acetone, ACS reagent, ≥99.5%
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Diethyl ether, ACS reagent, ≥98.0%, contains ≤2% ethanol and ≤10ppm BHT as inhibitor
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Methanol, ACS reagent, ≥99.8%
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Chloroform, ReagentPlus®, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Methanol, Absolute - Acetone free
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Methanol, ACS spectrophotometric grade, ≥99.9%
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Acetone, ACS reagent, ≥99.5%
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Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%
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Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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1-Octanol, ReagentPlus®, 99%