A rapid and sensitive assay for determination of doxycycline using thioglycolic acid-capped cadmium telluride quantum dots.

A rapid and sensitive assay for determination of doxycycline using thioglycolic acid-capped cadmium telluride quantum dots.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2015-07-24)

Javad Tashkhourian, Ghodratollah Absalan, Marzieh Jafari, Saber Zare

PMID26204505

ABSTRACT

A rapid, simple and inexpensive spectrofluorimetric sensor for determination of doxycycline based on its interaction with thioglycolic acid-capped cadmium telluride quantum dots (TGA/CdTe QDs) has been developed. Under the optimum experimental conditions, the sensor exhibited a fast response time of <10s. The results revealed that doxycycline could quench the fluorescence of TGA/CdTe QDs via electron transfer from the QDs to doxycycline through a dynamic quenching mechanism. The sensor permitted determination of doxycycline in a concentration range of 1.9×10(-6)-6.1×10(-5)molL(-1) with a detection limit of 1.1×10(-7)molL(-1). The sensor was applied for determination of doxycycline in honey and human serum samples.

MATERIALS

Product Number

Brand

Product Description

608777

Sigma-Aldrich

Ammonia-¹⁴N, 99.99 atom % ¹⁴N

632287

Sigma-Aldrich

VenPure^® SF, powder

W530574

Sigma-Aldrich

Hydrochloric acid solution, 32 wt. % in H₂O, FCC

W290017

Sigma-Aldrich

Phosphoric acid solution, 85 wt. % in H₂O, FCC, FG

612162

Sigma-Aldrich

Phosphoric acid solution, suitable for NMR (reference standard), 85% in D₂O (99.9 atom % D), NMR tube size 4.2 mm × 8 in. , WGS-5BL Coaxial NMR tube

779423

Sigma-Aldrich

Ammonia solution, 4 M in methanol

718939

Sigma-Aldrich

Ammonia solution, 0.4 M in THF

451010

Sigma-Aldrich

Potassium bromide, anhydrous, powder, 99.95% trace metals basis

229873

Sigma-Aldrich

Sodium acetate, 99.995% trace metals basis

449962

Sigma-Aldrich

Potassium bromide, anhydrous, powder, 99.999% trace metals basis

452378

Sigma-Aldrich

Tellurium, shot, ≤2 mm, 99.999% trace metals basis

263303

Sigma-Aldrich

Tellurium, granular, −5-+50 mesh, 99.99% trace metals basis

266418

Sigma-Aldrich

Tellurium, powder, −200 mesh, 99.8% trace metals basis

451029

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

282979

Sigma-Aldrich

3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%

71183

Sigma-Aldrich

Sodium acetate, anhydrous, BioUltra, suitable for luminescence, Molecular Biology, ≥99.0% (NT)

60128

Sigma-Aldrich

Potassium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

71196

Sigma-Aldrich

Sodium acetate solution, BioUltra, Molecular Biology, ~3 M in H₂O

409316

Sigma-Aldrich

Potassium chloride, 99.999% trace metals basis

426148

Sigma-Aldrich

Boric acid-¹¹B, ≥99 atom % ¹¹B

W302406

Sigma-Aldrich

Sodium Acetate Anhydrous, >99%, FG

204110

Sigma-Aldrich

Potassium nitrate, 99.99% trace metals basis

542040

Sigma-Aldrich

Potassium nitrate, 99.999% trace metals basis

528056

Sigma-Aldrich

Thioglycolic acid, ≥99%

449989

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

60089

Sigma-Aldrich

Potassium bromide, BioUltra, ≥99.5% (AT)

229938

Sigma-Aldrich

Sodium nitrate, 99.995% trace metals basis

72068

Sigma-Aldrich

Sodium hydroxide solution, BioUltra, Molecular Biology, 10 M in H₂O

71687

Sigma-Aldrich

Sodium hydroxide, BioUltra, suitable for luminescence, ≥98.0% (T), pellets

P6083

Sigma-Aldrich

Potassium nitrate, BioXtra, ≥99.0%