RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
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General description
Learn more about this product and view application data.
Application
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Legal Information
Kit Components Also Available Separately
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLSDS
recommended
required but not provided
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
WGK
WGK 3
Flash Point(F)
100.4 °F
Flash Point(C)
38 °C
Certificates of Analysis (COA)
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Articles
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protocols
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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