LC/MS/MS Analysis of Multiple Mycotoxins on Ascentis^® Express Phenyl-Hexyl

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

mobile phase

[A] 5mM ammonium formate with 0.1% formic acid in water ; [B] 5mM ammonium formate with 0.1% formic acid in acetonitrile

gradient

30 to 60% B in 3 min; to 100% B in 2 min; hold at 100% B for 2 min; 100 to 30% B in 0.5 min; hold at 30% for 2 min

flow rate

0.4 mL/min

pressure

5511 psi (380 bar)

column temp.

40 °C

detector

MS/MS, ESI(+), MRM (See Figure for transitions)

injection

2 μL