TAE Buffer, 10X, Molecular Biology Grade

Name: TAE Buffer, 10X, Molecular Biology Grade
Brand: MM
Price: 62.1 EUR
Availability: InStock

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Synonym(s):

Tris-Acetate-EDTA Buffer

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1 L

€62.10

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About This Item

UNSPSC Code:

12161700

NACRES:

NA.25

Key Documents

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form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
desiccated

technique(s)

electrophoresis: suitable

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

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This Item	574793	574795	PPB008
Sigma-Aldrich 574797 TAE Buffer, 10X, Molecular Biology Grade	Sigma-Aldrich 574793 TE Buffer, 100X, Molecular Biology Grade	Sigma-Aldrich 574795 TBE Buffer, 10X, Molecular Biology Grade	Sigma-Aldrich PPB008 Tris Acetate-EDTA buffer
technique(s) electrophoresis: suitable	technique(s) -	technique(s) -	technique(s) -
form liquid	form liquid	form liquid	form powder
storage condition OK to freeze, desiccated	storage condition OK to freeze, desiccated	storage condition OK to freeze	storage condition -
storage temp. 15-25°C	storage temp. 15-25°C	storage temp. 15-25°C	storage temp. -
foreign activity DNase, RNase, protease, none detected	foreign activity DNase, RNase, protease, none detected	foreign activity DNase, RNase, protease, none detected	foreign activity DNAse, none detected, Nickase, none detected, Protease, none detected, RNAse, none detected
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -

General description

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.[1]TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.[2]

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.[3]

Preparation Note

Dilute 1:10 with H₂O to prepare a 1X solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis

Sanderson BA, et al.

Analytical Biochemistry, 454, 44-52 (2014)

Design and assessment of engineered CRISPR-Cpf1 and its use for genome editing.

Bin Li et al.

Nature protocols, 13(5), 899-914 (2018-04-07)

Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both

Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis.

Brian A Sanderson et al.

Analytical biochemistry, 454, 44-52 (2014-03-19)

Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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