MAB2074Z

Anti-Integrin αVβ6 Antibody

CHEMICON^®, mouse monoclonal, E7P6

• Synonym(s): MAB2074
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• Product Name: Anti-Integrin αVβ6 Antibody, clone E7P6, azide free, clone E7P6, Chemicon^®, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: E7P6, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• isotype: IgG1
• suitability: not suitable for activity/function inhibition; not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_002210.2
• UniProt accession no.: P06756
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGAV(3685)

Description

Immunogen

Recombinant secreted human alphavbeta6.

Application

Immunohistochemistry (acetone fixation): Effective for human tissue, especially in circumstances where up-regulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alpavbeta6 in epithelia of healthy adults.

Immunocytochemistry: Effective for F6, HT-29, H441 cell lines (others not tested).

Flow cytometry: Effective for detection of human and monkey alphaVbeta6

Not effective for immunoprecipitation or western blot.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin αVβ6 Antibody, clone E7P6, azide free is validated for use in FC, IC, IH for the detection of Integrin αVβ6.

Biochem/physiol Actions

Monoclonal antibody MAB2074Z, raised by immunizing Balb/c mice with recombinant secreted human avb6, is useful for flow cytometry and is especially useful for immunocytochemistry. The antibody works well for immunohistochemistry of human tissue, especially under circumstances where upregulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alphavbeta6 in epithelia of healthy adults. This antibody does not recognize the alphaV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.

Physical form

Format: Purified

Sterile-filtered liquid at 1 mg/mL in PBS, containing no preservatives.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable