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Merck

PLHK07610

Regenerated Cellulose Membrane Filter, 100 kDa NMWCO

Ultracel®, filter diam. 76 mm

Synonym(s):

Ultrafiltration Discs, 100 kDa NMW, Ultrafiltration membrane discs

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About This Item

UNSPSC Code:
41104903
eCl@ss:
32031602
NACRES:
NB.24

Key Documents

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material

regenerated cellulose membrane

Quality Level

400

sterility

non-sterile

feature

hydrophilic

manufacturer/tradename

Ultracel®

technique(s)

protein clean-up: suitable (desalting)
protein purification: suitable (concentration)

diam.

76 mm

filter diam.

76 mm

filtration area

41.8 cm2

matrix

Ultracel®

pore size

100 kDa NMWCO

shipped in

ambient

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General description

Ultrafiltration membranes are generally composed of cellulose or polyethersulfone (PES). The material of choice will greatly depend on sample compatibility and the type of membrane will have a significant impact on performance. The molecular weight (MW) of the macrosolute that is to be retained determines the membrane nominal molecular weight cut-off (NMWCO). The diafiltration technique utilizes ultrafiltration membranes, either in centrifugal or pressure-driven devices such as the Amicon® Stirred Cell for efficient buffer exchange. The ultrafiltration membranes are preferred over dialysis tubing as the membranes can be used for both sample concentration and buffer exchange. This minimizes sample transfers and reduces sample loss.

Application

Ultrafiltration Discs, 100 kDa NMW has been used:
  • in Millipore stirred ultrafiltration cell (MSUC) for glucose/ gluconic acid (GA) conversion catalyzed by glucose oxidase (GO)
  • with Amicon® stirred cell model 8200 for microbial extracellular vesicles (EVs) isolation of large volumes cultures
  • in the synthesis of polydopamine (pDA)-modified iron oxide core-shell nanoparticles (IONPs)

Other Notes

Replaces: 14442AM

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ruihua Li et al.
Chemosphere, 243, 125391-125391 (2019-11-30)
In this study, novel lignin-based flocculant (LBF) was prepared in recycling of papermaking sludge. And LBF combined with polyaluminum chloride (PAC) was used in the coagulation/ultrafiltration combined process. Effects of polymer types, pH and ultrafiltration time on the membrane fouling
Ester Junko Tomotani et al.
Applied biochemistry and biotechnology, 121-124, 149-162 (2005-05-27)
Glucose oxidase (GO) (EC 1.1.3.4) was used as catalyst for oxidizing glucose into gluconic acid utilizing a 10-mL Bioengineering Enzyme Membrane Reactor or a 400-mL Millipore Stirred Ultrafiltration Cell (MSUC) coupled with a Millipore UF membrane (cutoff of 100 kDa)
Nimisha Singh et al.
Nanomaterials (Basel, Switzerland), 9(2) (2019-01-27)
Polydopamine (pDA)-modified iron oxide core-shell nanoparticles (IONPs) are developed and designed as nanovectors of drugs. Reactive quinone of pDA enhances the binding efficiency of various biomolecules for targeted delivery. Glutathione disulfide (GSSG), an abundant thiol species in the cytoplasm, was
Carolina Coelho et al.
Bio-protocol, 10(2), e3502-e3502 (2020-01-20)
Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix components and even nucleic acids and metabolites.

Related Content

Ultrafiltration Membranes
Amicon®Stirred Cell

The best in large-volume ultrafiltration just got better. Introducing the new Amicon® Stirred Cell. You’re an Amicon® Stirred Cell owner. You’re adept at finely separating macrosolutes in large-volume (50-400 mL) samples, and you depend on the precision of Amicon® devices. Or maybe you are an expert in membrane analysis, and you count on a device that lets you insert membranes of your choice. With your needs in mind, EMD Millipore has developed the new Amicon® Stirred Cell, providing the same gentle, high recovery of macrosolutes and thorough buffer exchange to which you’re well accustomed. It provides the same membrane flexibility and ability to monitor filtration progress.

Amicon® Stirred Cell enables gentle and efficient large volume sample concentration

The Amicon® Stirred Cell family of pressure-driven filtration devices provide an ideal solution for concentrating and buffer-exchanging large volumes of macrosolutes. These devices are available in multiple sizes to offer a wide range of processing volumes. Additionally, to accommodate expansion to even larger processing volumes, an external reservoir can be attached to any Amicon® Stirred Cell. Unlike ultrafiltration with tangential flow filtration (TFF) devices, which are manufactured with pre-sealed membranes, the Amicon® Stirred Cells use membrane discs, which are easily exchanged to match changing sample preparation needs.

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