Roche

DNase I

Name: DNase I
Brand: ROCHE
Price: 116 EUR

grade II, from bovine pancreas

Synonym(s):

Deoxyribonuclease, Deoxyribonucleate 5′-oligonucleotido-hydrolase

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100 MG

€116.00

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About This Item

UNSPSC Code:

12352204

Key Documents

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biological source

bovine pancreas

Quality Level

100

form

lyophilized

packaging

pkg of 100 mg

manufacturer/tradename

Roche

technique(s)

DNA extraction: suitable

optimum pH

~7.0

storage temp.

2-8°C

Related Categories

Nucleases (DNases and RNases)

Proteins & Enzymes

Roche Life Science Products

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This Item	11284932001	D7291	AMPD1
Roche 10104159001 DNase I	Roche 11284932001 DNase I	Sigma-Aldrich D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas	Sigma-Aldrich AMPD1 DNase I
technique(s) DNA extraction: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) DNA extraction: suitable	technique(s) RT-PCR: suitable
biological source bovine pancreas	biological source bovine pancreas	biological source bovine pancreas	biological source -
form lyophilized	form lyophilized	form buffered aqueous glycerol solution	form liquid
optimum pH ~7.0	optimum pH -	optimum pH -	optimum pH -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
Quality Level 100	Quality Level 100	Quality Level 200	Quality Level 200

General description

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.[2]

Application

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-

The isolation of cells from lung, skin and tumor samples[3][4][5][6]
Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

Preparation Note

Activator: Ca²⁺ (0.12 mM) in combination with Mg²⁺ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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