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Merck

A9576

Bovine Serum Albumin solution

30% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Bovine Serum Albumin solution

Synonym(s):

Albumin solution from bovine serum, BSA solution

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50 ML

€609.00

€609.00


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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75

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biological source

bovine

sterility

sterile-filtered

product line

BioXtra

Assay

28-32% total protein basis (UV)

form

liquid

origin

USA origin

concentration

in phosphate buffer (DPBS)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile on Raw material, none detected

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
A7979A8412A1595
origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

technique(s)

cell culture | mammalian: suitable

technique(s)

blocking: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

28-32% total protein basis (UV)

assay

33-37% total protein basis (UV)

assay

7.0-8.0% protein basis (UV)

assay

90-120 mg/mL protein basis (biuret)

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

Quality Level

300

Quality Level

400

Quality Level

200

Quality Level

300

form

liquid

form

liquid

form

liquid

form

solution

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

BSA 30% solution is a convenient form for direct supplementation of serum-free media. Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Preparation Note

Prepared from A9647 in DPBS (D5652).

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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J A A Pires et al.
Journal of dairy science, 102(6), 5347-5360 (2019-03-25)
The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows. Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for
Paul W Kriebel et al.
The Journal of cell biology, 217(8), 2891-2910 (2018-06-10)
Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
David G Courtney et al.
Cell host & microbe, 22(3), 377-386 (2017-09-15)
Many viral RNAs are modified by methylation of the N

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Questions

1–2 of 2 Questions  
  1. Is this product prepared in DPBS with or without Ca++ and Mg++?

    1 answer
    1. A9576 is prepared with modified DPBS without calcium chloride and magnesium chloride.

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  2. What is the open stability of A9576?

    1 answer
    1. This product is assigned a recommended retest date of approximately 3 years from the date of Quality Release. Provided that the product is stored properly and no contaminants have been introduced, once opened, the product is expected to be stable up to the lot-specific retest date. The retest date is published in the product Certificate of Analysis. Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a9576#product-documentation

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