C8302

4-Chloro-1-naphthol solution

Name: 4-Chloro-1-naphthol solution
Brand: SIGMA
Price: 109 EUR

For HRP detection on Western Blots

Slide 1 of 1

Select a Size

100 ML

€109.00

Please contact Customer Service for Availability

About This Item

CAS Number:

604-44-4

NACRES:

NA.52

PubChem Substance ID:

24893047

UNSPSC Code:

12352200

MDL number:

MFCD00003974

Key Documents

View All Documentation

Product Name

4-Chloro-1-naphthol solution, For HRP detection on Western Blots

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

grade

Molecular Biology

form

liquid

storage temp.

2-8°C

Quality Level

200

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	N7660	185507	N2125
Sigma-Aldrich C8302 4-Chloro-1-naphthol solution	Sigma-Aldrich N7660 4-Nitrophenol solution	Sigma-Aldrich 185507 2-Naphthol	Sigma-Aldrich N2125 Naphthol AS-BI phosphate
grade for molecular biology	grade -	grade -	grade -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form liquid	form liquid	form powder or flakes	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. −20°C

Application

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Biochem/physiol Actions

Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.

General description

4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.

Other Notes

0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

related product

Product No.

Description

Pricing

P7170

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

P7059

Phosphate Buffered Saline, 10× PBS for Western blots and IP

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

W0138

Western Blocker^™ Solution, for HRP detection systems

SDS7B2

Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da

C1992

ColorBurst^™ Electrophoresis Marker, mol wt 8,000-220,000 Da

GERPN2209

ECL^™ Western Blotting Detection Reagents, Cytiva RPN2209

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H312 + H332,H370

pcodes

P260 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P311

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

163.9 °F - closed cup

flash_point_c

73.3 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Enhanced monoterpene emission in transgenic orange mint (Mentha × piperita f. citrata) overexpressing a tobacco lipid transfer protein (NtLTP1).

Hwan-Su Hwang et al.

Planta, 252(3), 44-44 (2020-09-03)

Overexpression of the tobacco lipid transfer protein (NtLTP1) gene in transgenic orange mint resulted in enhanced accumulation of monoterpenes in the cavity of head cells of glandular trichomes, which resulted in enhanced emission of monoterpenes from transgenic orange mints. Plants

Role of PKCζ-NADPH oxidase signaling axis in PKCα-mediated Giα2 phosphorylation for inhibition of adenylate cyclase activity by angiotensin II in pulmonary artery smooth muscle cells.

Animesh Chowdhury et al.

Cell biology international, 44(5), 1142-1155 (2020-01-23)

We sought to determine the mechanism by which angiotensin II (AngII) inhibits isoproterenol induced increase in adenylate cyclase (AC) activity and cyclic adenosine monophosphate (cAMP) production in bovine pulmonary artery smooth muscle cells (BPASMCs). Treatment with AngII stimulates protein kinase

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service