The recommended storage temperature for this product is 2-8°C. While freezer storage is not required, storing this solution in frozen aliquots at -20°C will not impact the integrity of the protein. Avoid repeated freeze/thaw cycles. The long term stability of this material when stored frozen has not been evaluated and may not result in extended shelf life.
F1141
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
Synonym(s):
Fibronectin
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1 MG
€319.00
2 MG
€604.00
5 MG
€1,090.00
About This Item
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Product Name
Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture
biological source
bovine plasma
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
packaging
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
UniProt accession no.
Binding Specificity
Peptide Source: Collagen
Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... FN1(280794)
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1 of 4
This Item | F0895 | F0556 | 341631 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile | sterility sterile-filtered | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| biological source bovine plasma | biological source human plasma | biological source human fibroblasts | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form solution | form liquid | form solution | form liquid |
| binding specificity Peptide Source: Collagen | binding specificity Peptide Source: Collagen | binding specificity Peptide Source: Collagen | binding specificity - |
General description
Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences.[1] Fibronectins belongs to a class of high-molecular-weight glycoproteins.[2] Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.[1]
Application
Biochem/physiol Actions
Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.[2]
Analysis Note
Protein homogeneity is evaluated by immunoelectrophoresis.
Other Notes
Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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Can I freeze the aliquoted Fibronectin?
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Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!
1 answer-
The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f1141#product-documentationHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How long can I store the fibronectin solution?
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The product is good for at least a year at 2-8 °C if kept sterile.
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What is the procedure for coating culture surfaces with fibronectin?
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To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
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The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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How long can I store fibronectin coated plates, etc?
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Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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