As indicated above on this PDP, the immunogen for this polyclonal antibody is ferritin isolated from normal human spleen. Sequence information can be found in GenBank at the following link:
https://www.uniprot.org/uniprotkb/P02794/entry#sequences
F5012
Anti-Ferritin, Human antibody produced in rabbit
whole antiserum, liquid
Synonym(s):
Anti-FHC, Anti-FTH, Anti-FTHL6, Anti-HFE5, Anti-PIG15, Anti-PLIF
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biological source
rabbit
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
15 mM sodium azide
species reactivity
human
technique(s)
Ouchterlony double diffusion: 1:16
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FTH1(2495)
1 of 4
This Item | SAB1410457 | SAB1409042 | SAB1405830 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| antibody form whole antiserum | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| technique(s) Ouchterlony double diffusion: 1:16 | technique(s) western blot: 1 μg/mL | technique(s) western blot: 1 μg/mL | technique(s) western blot: 1 μg/mL |
| Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) | Gene Information human ... FTH1(2495) |
General description
Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.
Immunogen
Ferritin isolated from normal human spleen
Application
- Anti-Ferritin, Human antibody produced in rabbit has been used in:
- immunohistochemistry
- western blotting
- immunocytochemical techniques
Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.
Preparation Note
The antiserum has been treated to remove lipoproteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Scientific tests
Aldred EM, et al.
Pharmacology, 331-341 (2009)
Moon-Suhn Ryu et al.
The Journal of clinical investigation, 127(5), 1786-1797 (2017-04-05)
Developing erythrocytes take up exceptionally large amounts of iron, which must be transferred to mitochondria for incorporation into heme. This massive iron flux must be precisely controlled to permit the coordinated synthesis of heme and hemoglobin while avoiding the toxic
Zhao Wang et al.
International journal of hematology, 90(4), 501-505 (2009-08-06)
Elevated levels of serum ferritin and a low percentage of glycosylated ferritin have been reported in adult-onset Still's disease (AOSD) as well as in hemophagocytic lymphohistiocytosis (HLH). The objective of the current study was to investigate total ferritin levels and
Pro-inflammatory activation of microglia in the brain of patients with sepsis
Zrzavy T, et al.
Neuropathology and Applied Neurobiology, 45(3), 278-290 (2019)
Iron-Induced Retinal Damage
Handbook of nutrition, diet, and the eye, 619-626 (2014)
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