G7654
Gel Loading Solution
for NA electrophoresis, solution
Synonym(s):
DNA Gel Loading Solution, Gel Loading Buffer
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About This Item
UNSPSC Code:
12161703
NACRES:
NA.25
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This Item | G2526 | R1386 | R4268 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade for molecular biology | grade for molecular biology |
| concentration 6 × | concentration - | concentration - | concentration 1.25 × |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form liquid | form solution | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. −20°C |
General description
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
Preparation Note
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
Other Notes
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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M K Bolla et al.
Journal of lipid research, 40(12), 2340-2345 (1999-12-10)
The apoE gene exhibits two common polymorphisms that have been associated with both coronary artery disease and Alzheimer's disease. The polymorphisms create the three allelic isoforms E2, E3, and E4 which are encoded by Cys;-Cys, Cys;-Arg, and Arg;-Arg at amino
Jung-Lim Lee et al.
Journal of microbiological methods, 67(3), 456-462 (2006-12-22)
Ethidium bromide monoazide (EMA) was utilized to selectively allow conventional PCR amplification of target DNA from viable but not dead cells from a broth culture of bacterial mixed flora derived from cod fillets. The universal primers designated DG74 and RW01
Peter Konings et al.
Nature protocols, 7(2), 281-310 (2012-01-21)
We present a protocol for reliably detecting DNA copy number aberrations in a single human cell. Multiple displacement-amplified DNAs of a cell are hybridized to a 3,000-bacterial artificial chromosome (BAC) array and to an Affymetrix 250,000 (250K)-SNP array. Subsequent copy
S Henry et al.
Vox sanguinis, 70(1), 21-25 (1996-01-01)
While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
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