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Merck

906883

Decellularized ECM bioink precursor

from porcine bone, suitable for 3D bioprinting applications

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Product Name

Decellularized ECM bioink precursor, from porcine bone, suitable for 3D bioprinting applications

form

semisolid

composition

Collagen (hydroxyproline), 60-120 μg/mg
GAG (bicolor), 1.5-5.0 μg/mg

color

white to beige

storage temp.

−20°C

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Application

The extracellular matrix (ECM), comprised of proteins and other growth factors, plays a pivotal role in directing and mediating cellular activities in a tissue or an organ. Decellularized extracellular matrix (dECM) could not only replicate natural ECM microenvironment, but also provide tissue specific compositions and topology. dECM bioink is capable of providing optimized tissue-specific microenvironment conducive to the growth of 3D structured tissues.
Decellularized ECM bioink precursor is provided as an aseptic form. Reconstitution protocol is provided to prepare dECM bioink solution. The final bioink is used for bioprinting self-supporting 3D structures.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCR1385
MKCL0887

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Geunseon Ahn et al.
Scientific reports, 7(1), 8624-8624 (2017-08-19)
Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks
Byoung Soo Kim et al.
Biomaterials, 168, 38-53 (2018-04-04)
3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source
Deepak Choudhury et al.
Trends in biotechnology, 36(8), 787-805 (2018-04-22)
The extracellular matrix (ECM) comprises a complex milieu of proteins and other growth factors that provide mechanical, biophysical, and biochemical cues to cells. The ECM is organ specific, and its detailed composition varies across organs. Bioinks are material formulations and
Falguni Pati et al.
Nature communications, 5, 3935-3935 (2014-06-03)
The ability to print and pattern all the components that make up a tissue (cells and matrix materials) in three dimensions to generate structures similar to tissues is an exciting prospect of bioprinting. However, the majority of the matrix materials

Articles

Three-Dimensional Bioprinting for Tissue and Disease Modeling

Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.

Protocols

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