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G0799

β-Glucuronidase from Escherichia coli

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
EC Number:
3.2.1.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00199013
UNSPSC Code:
12352204
NACRES:
NA.54

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recombinant

expressed in E. coli

form

aqueous glycerol solution

specific activity

>20,000,000 units/g protein

mol wt

74 kDa

concentration

>1 mg/mL

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
G8162G8420G8295
β-Glucuronidase from Escherichia coli >20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Sigma-Aldrich

G0799

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)

Sigma-Aldrich

G8162

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8420

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8295

β-Glucuronidase from Escherichia coli

specific activity

>20,000,000 units/g protein

specific activity

≥5,000,000 units/g protein, pH 6.8 (biuret)

specific activity

≥20,000 units/mg protein

specific activity

≥10,000,000 units/g protein (30 min assay)

concentration

>1 mg/mL

concentration

-

concentration

-

concentration

-

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli overproducing strain

recombinant

expressed in E. coli overproducing strain

form

aqueous glycerol solution

form

aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

mol wt

74 kDa

mol wt

69-71 kDa

mol wt

290 kDa

mol wt

~290 kDa

General description

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.[1]

Application

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.[2]
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLBB6139V

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E M Schmelz et al.
Cancer research, 59(22), 5768-5772 (1999-12-03)
Dietary sphingolipids inhibit chemically induced colon cancer in mice. The most likely mediators of this effect are the metabolites ceramide (Cer) and sphingosine, which induce growth arrest and apoptosis in transformed cells. Sphingolipids are digested in both the upper and
D H Kim et al.
Biological & pharmaceutical bulletin, 18(9), 1184-1188 (1995-09-01)
beta-Glucuronidase was purified 360-fold from Escherichia coli HGU-3, an human intestinal bacterium. The specific activity of the purified enzyme was 17.78 units/mg protein. The enzyme (M.W. 290000) is composed of four subunits (M.W. 72000) with a pI and optimal pH
Fei Song et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 67(11-12), 611-619 (2013-02-19)
Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements
Xiaoyan Liu et al.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 42(1), 67-74 (2013-03-19)
To construct a lentiviral RNA interference system targeting heparanase (HPSE) based on miR30 and to test its silencing effect. Three heparanase-shRNA structures were designed based miR30. The targeting fragments were obtained by PCR, then inserted into the vector LV PP-GFP
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
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Protocols

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

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