biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG
storage temp.
2-8°C
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
Paxillin is a focal adhesion adaptor protein associated with th establishment of focal adhesions, cell signaling via integrins, and cell motility. The N-terminus of paxillin contains a proline-rich region for SH3 and SH2 domain binding. The N-terminus also contains five copies of the LD motif for association of actin binding proteins. The C-terminus contains LIM domains and the focal adhesion targeting sequence. Paxillin is believed to function as an adaptor protein that facilitates assembly of multi-protein complexes and coordinates signaling via integrins. Tyrosine and serine phosphorylation of paxillin is associated with the establishment of cytoskeletal membrane attachments. Phosphorylation by JNK at Ser178 is necessary for normal cell motility.
Recognizes the ~68 kDa paxillin protein phosphorylated at Ser178.
This PhosphoDetect Anti-Paxillin (pSer¹⁷⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of Paxillin (pSer¹⁷⁸).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁷⁸ phosphorylation site of human paxillin
Application
Immunoblotting (1:1000-1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In Tris-citrate/phosphate, pH 7.0-8.0.
Preparation Note
Following initial use aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cylces.
Analysis Note
Positive Control
NIH3T3 cells plated on fibronectin
NIH3T3 cells plated on fibronectin
Other Notes
Bellis, S.L., et al. 1997. Biochem. J.325, 375.
Schaller, M.D. 2001. Oncogene20, 6459.
Huang, C., et al. 2003. Nature424, 219.
Schaller, M.D. 2001. Oncogene20, 6459.
Huang, C., et al. 2003. Nature424, 219.
Does not recognize unphosphorylated paxillin. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Highly Toxic (H)
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저장 등급
12 - Non Combustible Liquids
WGK
WGK 1
인화점 (F)
Not applicable
인화점 (°C)
Not applicable