recombinant
expressed in Pichia pastoris
assay
95% (gel filtration)
form
lyophilized
specific activity
≥150 units/mg protein
packaging
pkg of 4 × 100 μg (03708969001), pkg of 4 × 25 μg (03708985001)
manufacturer/tradename
Roche
storage temp.
−20°C
General description
This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.
Application
Biochem/physiol Actions
Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.
Preparation Note
Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!
Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.
Other Notes
For life science research only. Not for use in diagnostic procedures.
경고어
Danger
표적 장기
Respiratory system
저장 등급
11 - Combustible Solids
무슨 일이 일어나고 있는 걸까?
WGK 1
인화점 (F)
does not flash
인화점 (°C)
does not flash
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3