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이 품목 | 4374771 | 4381662 | 4369561 |
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Analysis Note
To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.
To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.
To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.
Application
iTRAQ® Reagent - 8PLEX Multiplex Kit has been used:
- to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteome[1]
- in isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptides[2]
- as isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis[3]
General description
The isobaric tags for relative and absolute quantitation (iTRAQ®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs.
Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.
Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry® product number 4390812.
Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry® product number 4390812.
Legal Information
iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.
Features and Benefits
- Simultaneously identify and quantify proteins from multiple samples
- Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
- Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
- Increases throughput and confidence in results for protein biomarker discovery studies
- Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
- Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
- Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
저장 등급
3 - Flammable liquids
flash_point_f
35.6 °F - closed cup
flash_point_c
2.0 °C - closed cup
Juan Jin et al.
Biomarkers in medicine, 14(10), 839-854 (2020-08-29)
Background: Proteome studies for multiple renal diseases is bare. Methodology & results: Using isobaric tags for relative and absolute quantitation labeling, many differentially expressed proteins (DEPs) were identified in acute kidney injury (AKI), AKI + chronic kidney disease (CKD), diabetic CKD and
Jesús Alejandro Zamora-Briseño et al.
Frontiers in microbiology, 11, 1084-1084 (2020-06-18)
The Caribbean spiny lobster Panulirus argus (Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virus Panulirus argus Virus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile
He Wang et al.
Journal of cellular physiology, 234(8), 12897-12909 (2018-12-18)
Our previous study showed that knocking down integrin α5 (ITGA5) expression by using a lentiviral vector in human dental pulp stem cells (DPSCs) led to weakening proliferation and migration capacity while enhanced odontogenic differentiation. To seek for possible clinical application
He Wang et al.
Archives of oral biology, 89, 9-19 (2018-02-07)
This study aimed to compare the proteomic profiling of human dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) under in vitro osteogenic induction, which imitates the microenvironment during osteo-/odontogenesis of DPSCs and PDLSCs. The proteomic profiles of
Shaoli Liu et al.
Journal of microbiology and biotechnology, 30(2), 187-195 (2019-11-23)
To understand the molecular mechanism involved in the survivability of cold-tolerant lactic acid bacteria was of great significance in food processing, since these bacteria play a key role in a variety of low-temperature fermented foods. In this study, the cold-stress
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