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76216

Papain from Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

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CAS 번호:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
EC 번호:
Specific activity:
≥10 U/mg protein (~25 mg/ml)
Biological source:
papaya
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biological source

papaya

Quality Segment

form

solution

specific activity

≥10 U/mg protein (~25 mg/ml)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

color

light brown

storage temp.

2-8°C

General description

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.

Application

Papain from Carica papaya has been used:

  • in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
  • to study its effects on Alpaca seminal plasma viscosity and sperm function
  • to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

유사한 품목 비교

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차이점 표시

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이 품목
P5306P31251495005
USP

USP

1495005

Papain

description

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

description

lyophilized powder, aseptically filled

description

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

description

United States Pharmacopeia (USP) Reference Standard

biological source

papaya

biological source

papaya (latex)

biological source

-

biological source

-

specific activity

≥10 U/mg protein (~25 mg/ml)

specific activity

≥8.0 units/mg protein

specific activity

≥16 units/mg protein

specific activity

-

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

solution

form

lyophilized powder

form

buffered aqueous suspension

form

solid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

color

light brown

color

-

color

-

color

-


픽토그램

Health hazardExclamation mark

경고어

Danger

위험 코드

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 장기

Respiratory system

저장 등급

11 - Combustible Solids

무슨 일이 일어나고 있는 걸까?

WGK 1

인화점 (F)

Not applicable

인화점 (°C)

Not applicable

개인 보호 장비(PPE)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Lot/Batch Number

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질문

  1. Which solution is used in Papain from Carica papaya solution? DMSO? water?

    1 답변
    1. This product is an aqueous solution containing 35 - 55% glycerol.

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