Application
This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.
This kit contains sufficient reagents for one 96 well plate (96 tests)
1 of 1
이 품목 | |||
|---|---|---|---|
| description - | description Ex: 320nm, Em: 420nm | description high sensitivity assay | description clone 1696, purified immunoglobulin, buffered aqueous solution |
| form solution | form lyophilized powder | form - | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
인화점 (F)
Not applicable
인화점 (°C)
Not applicable
저장 등급
12 - Non Combustible Liquids



