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M3003

Myokinase from rabbit muscle

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

동의어(들):

ATP: AMP phosphotransferase, Adenylate Kinase

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크기 선택

보기 변경
사이즈/SKU재고 정보가격 (VAT 별도)
1000 units
구입 가능 여부는 고객센터에 문의하십시오.
₩164,430
2500 units
구입 가능 여부는 고객센터에 문의하십시오.
₩263,291
5000 units
구입 가능 여부는 고객센터에 문의하십시오.
₩453,502
10000 units
구입 가능 여부는 고객센터에 문의하십시오.
₩751,506

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-743-3
NACRES:
NA.54
MDL number:
EC 번호:
Specific activity:
1,500-3,000 units/mg protein (biuret)
Biological source:
rabbit muscle

₩164,430


구입 가능 여부는 고객센터에 문의하십시오.

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biological source

rabbit muscle

Quality Segment

form

ammonium sulfate suspension

specific activity

1,500-3,000 units/mg protein (biuret)

mol wt

21 kDa

foreign activity

3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%

storage temp.

2-8°C

General description

Research area: Cell Signaling
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.

Application

Myokinase has been used:

  • in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate
  • in a study to investigate the ligand-binding properties of its peptide fragments
  • as one of the components in the ATP generation solution for the quantification of 3’3’-c-di-AMP using an AMP-Luciferase assay

Biochem/physiol Actions

Myokinase or adenylate kinases, play a crucial role in regulating energy metabolism within cells and balancing adenine nucleotide ratios in different cellular compartments.

Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0

Other Notes

One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.

유사한 품목 비교

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차이점 표시

1 of 1

이 품목
475941C3755P1506
description

ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)

description

-

description

Type I, salt-free, lyophilized powder, ≥150 units/mg protein

description

Type II, ammonium sulfate suspension, 350-600 units/mg protein

biological source

rabbit muscle

biological source

-

biological source

-

biological source

rabbit muscle

specific activity

1,500-3,000 units/mg protein (biuret)

specific activity

≥10 units/mg solid, ≥200 U/mg

specific activity

≥150 units/mg protein

specific activity

350-600 units/mg protein

form

ammonium sulfate suspension

form

lyophilized

form

salt-free, lyophilized powder

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

21 kDa

mol wt

-

mol wt

80-86.2 kDa

mol wt

237 kDa

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200


저장 등급

10 - Combustible liquids

WGK

WGK 3

인화점 (F)

Not applicable

인화점 (°C)

Not applicable

개인 보호 장비(PPE)

Eyeshields, Gloves, type N95 (US)



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시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

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질문

  1. M3003-1KUの容量についてお教えください。

    1 답변
    1. This product is an ammonium sulfate suspension of Myokinase. This information may be determined by values reported in the Certificate of Analysis. A survey of recent lots indicates the an average volume of 160 uL for the 1KU package size, 400 uL for the 2.5KU package size, 800 uL for the 5KU package size, and 1.6 mL for the 10KU package size. The enzyme activity may range from 1500 - 3000 units per milligram protein and the protein concentration may range from 1 - 10 mg/mL. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/m3003#product-documentation

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