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P1435

Phosphatase, Acid from sweet potato

Name: Phosphatase, Acid from sweet potato
Brand: SIGMA
Price: 745213 KRW

ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)

동의어(들):

(Orthophosphoric-monoester phosphohydrolase [acid optimum] )

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보기 변경

사이즈/SKU	재고 정보	가격 (VAT 별도)
500 units	구입 가능 여부는 고객센터에 문의하십시오.	₩745,213
2500 units	구입 가능 여부는 고객센터에 문의하십시오.	₩3,143,760

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9001-77-8

NACRES:

NA.54

UNSPSC Code:

12352204

EC Number:

232-630-9

MDL number:

MFCD00131847

EC 번호:

3.1.3.2 (BRENDA, IUBMB)

eCl@ss:

42010102

Specific activity:

≥10.0 units/mg protein (modified Warburg-Christian)

₩745,213

구입 가능 여부는 고객센터에 문의하십시오.

For a suitable alternative that ships today, try P3752-100MG.

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도움 문의

속성

form

ammonium sulfate suspension

Quality Segment

300

specific activity

≥10.0 units/mg protein (modified Warburg-Christian)

secondary activity

≤9.9 units/mg protein ATPase

mol wt

110 kDa

storage temp.

2-8°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

설명

General description

Acid phosphatase from sweet potato is a phosphomonoesterase, which can appear in multiple molecular forms of similar molecular mass but with different isoelectric points.

Application

Potato acid phosphatase is used as a dephosphorylating reagent in thiochrome assays for the detection of thiamin in biological samples. In this assay, it was shown to be more effective than acid phosphatases from wheat germ or α-amylase.

Sweet potato acid phosphatase has been used in a study as a pre-column enzyme reactor via a covalent bond with glutaraldehyde and aminopropyl controlled-pore glass. It has also been used in a study to investigate the use of tyrosine, histidine, and cysteine as ligands for Mn(III) in sweet potato phosphatase.

Biochem/physiol Actions

Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: APaseR-PO₄ + H₂O --------------- R-OH + HOPO₃ ²⁺Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transduction processes of the cell. As a group, Apases may be as important as kinases in regulatory processes.

Physical form

Suspension in 1.8 M (NH₄)₂SO₄, 10 mM MgCl₂, pH 5.3

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

유사한 품목 비교

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차이점 표시

1 of 1

이 품목	SRE0048	P3752	P1146
Sigma-Aldrich P1435 Phosphatase, Acid from sweet potato	Sigma-Aldrich SRE0048 Phosphatase, Acid from potato	Sigma-Aldrich P3752 Phosphatase, Acid from potato	Sigma-Aldrich P1146 Phosphatase, Acid from potato
description ammonium sulfate suspension, ≥10.0 units/mg protein (modified Warburg-Christian)	description Suitable for manufacturing of diagnostic kits and reagents	description lyophilized powder, ≥0.5 unit/mg solid	description lyophilized powder, ≥3.0 units/mg solid
specific activity ≥10.0 units/mg protein (modified Warburg-Christian)	specific activity 3.0-10.0 unit/mg solid	specific activity ≥0.5 unit/mg solid	specific activity ≥3.0 units/mg solid
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 110 kDa	mol wt -	mol wt 69 kDa	mol wt 69 kDa
Quality Level 300	Quality Level 400	Quality Level 200	Quality Level 300
secondary activity ≤9.9 units/mg protein ATPase	secondary activity -	secondary activity -	secondary activity -

픽토그램

GHS08

경고어

Danger

위험 코드

H334

주의 사항

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

저장 등급

10 - Combustible liquids

WGK

WGK 3

인화점 (F)

Not applicable

인화점 (°C)