05-368

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

clone MPM-2, Upstate^®, from mouse

• Synonym(s): Phospho-Ser/Thr Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702

Properties

• Product Name: Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, clone MPM-2, Upstate^®, from mouse
• biological source: mouse
• antibody form: purified immunoglobulin
• clone: MPM-2, monoclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer/pThr)
• Quality Level: 100

Description

Analysis Note

Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde

routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells

Application

Anti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Recognizes phosphorylated serines or threonines when followed by a proline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This antibody recognizes a variety of proteins that are phosphorylated during mitosis.

Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.

varies depending on the protein you are detecting

Immunogen

Mitotic human HeLa cell cytosolic lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1