This product has not been tested for any specific application, so a specific protocol is not available. However, this material has been used successfully by a number of researchers.
Please see the link below to review publications that may be helpful:
https://www.sigmaaldrich.com/search/287810?focus=papers&page=1&perpage=30&sort=relevance&term=287810&type=citation_search
287810
2′,7′-Dichlorofluorescin Diacetate
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
Synonym(s):
2′,7′-Dichlorofluorescin Diacetate, H2DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
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About This Item
Empirical Formula (Hill Notation):
C24H16Cl2O7
CAS Number:
Molecular Weight:
487.29
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
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Product Name
2′,7′-Dichlorofluorescin Diacetate, Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
SMILES string
Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
assay
≥94% (HPLC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
Quality Level
Related Categories
1 of 4
This Item | D6883 | 35845 | 21884 |
|---|---|---|---|
| assay ≥94% (HPLC) | assay ≥97% | assay ≥95% (HPLC) | assay ≥85% (HPCE) |
| form crystalline solid | form powder | form - | form - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility DMSO: 5 mg/mL | solubility - | solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble, methanol: soluble |
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Product does not compete with ATP.
Reversible: no
Disclaimer
Toxicity: Standard Handling (A)
General description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.
Other Notes
Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
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