DP02A

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate

slurry (Liquid), clone PAb416, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: PAb416, monoclonal
• Species reactivity: SV40-infected cells
• Application: —
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PAb416, monoclonal
• form: slurry (Liquid)
• contains: ≤0.1% sodium azide as preservative
• species reactivity: SV40-infected cells
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoprecipitation (15 µL suspension/sample); Affinity Purification )
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Supplied as an agarose conjugate. Recognizes the ~94 kDa SV40 large T antigen.

Recognizes the ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Cat. No. DP02A is linked to agarose for immunoprecipitation and purification.

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Agarose Conjugate is validated for use in Immunoprecipitation, Affinity Purification for the detection of SV40 T Antigen (Ab-2).

Immunogen

purified SV40 large T-antigen

Epitope: Within amino acids 83-128

Application

Immunoprecipitation (15 µl suspension/sample)

Affinity Purification (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

50% slurry in PBS.

Analysis Note

Positive Control
SV80 or SV-T2 cells

Other Notes

DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P., 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10,

Suitable for affinity purification and immunoprecipitation. The amount of suspension required for purification is dependent on individual sample types.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable