MB000000T

Tryptone Glucose Extract Broth (Dehydrated)

powder (dehydrated), for heterotrophic bacteria, pkg of 110 g, suitable for microbiology

• Synonym(s): TGE broth, Tryptone Glucose Yeast Extract Broth
• UNSPSC Code: 41106213
• NACRES: NA.74
• eCl@ss: 32160801
• Ph: 7.0±0.2 (25.0 °C)
• Form: powder (dehydrated)
• Suitability: Aspergillus niger, Bacillus subtilis, Candida albicans, Clostridium sporogenes, Pseudomonas aeruginosa, Staphylococcus auerus, heterotrophic bacteria
• Application(s): clinical; cosmetics; environmental; food and beverages; industrial qc; water monitoring, microbiology

Properties

• Quality Segment: 100
• description: A non-selective medium used to detect total heterotrophic microorganisms in water and other liquids
• form: powder (dehydrated)
• shelf life: 60 mo.
• packaging: pkg of 110 g
• color: medium amber prepared medium, light tan dehydrated powder
• input: sample type beer; sample type cosmetics; sample type wine; sample type beverage(s); sample type food(s); sample type soft drink; sample type sport drink; sample type bottled water; sample type cider
• pH: 7.0±0.2 (25.0 °C)
• application(s): clinical; cosmetics; environmental; food and beverages; industrial qc; water monitoring, microbiology
• compatibility: for use with MF-method, for use with Microfil^®, for use with MicropreSure^®, for use with Milliflex^®
• shipped in: ambient
• storage temp.: 2-30°C
• suitability: Aspergillus niger, Bacillus subtilis, Candida albicans, Clostridium sporogenes, Pseudomonas aeruginosa, Staphylococcus auerus, heterotrophic bacteria

Description

General description

Tryptone Glucose Extract Broth is a non-selective microbiological medium designed for the detection and enumeration of total heterotrophic microorganisms in water, dairy products, and other liquids such as beer, bottled water, cider, soft drinks, sport drinks, and wine. It is available as a dehydrated powder and contains tryptone and yeast extract, which provide essential nitrogenous and carbonaceous compounds, amino acids, and vitamin B complex for microbial growth. Glucose serves as the primary energy source. The medium has a pH of 7.0 ± 0.2 at 25°C and is compatible with various filtration methods.

Application

Tryptone Glucose Extract Broth is widely used for the enumeration of heterotrophic bacteria in water quality testing, dairy product analysis, and microbial monitoring in beverages. It is also applied in bioburden testing across clinical, cosmetic, environmental, food and beverage, and industrial quality control. Additionally, it is utilized for detecting thermophilic bacteria in milk and dairy products to identify heat-resistant microorganisms.

Type of Test
Bioburden

Microbial Limits

Features and Benefits

Incubation Temperature: 28–35 °C

Incubation Time: 18–120 hrs

Packaging

Dehydrated 110 g in plastic bottle

Preparation Note

Tryptone Glucose Extract

Analysis Note

Colonies appear clear to creamy white, some may produce pigment

Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3