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PC548
Anti-Porin (Ab-5) (185-197) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-VDAC (Voltage-Dependent Anion Channel)
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat
Application:
—
Citations:
19
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VDAC1(7416)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~31 kDa (VDAC) porin protein.
Recognizes the ~31 kDa porin protein.
This Anti-Porin (Ab-5) (185-197) Rabbit pAb is validated for use in Immunoblotting for the detection of Porin (Ab-5) (185-197).
Immunogen
Human
a synthetic peptide corresponding to amino acids 185-197 of human porin
Application
Immunoblotting (2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Rat heart tissue
Rat heart tissue
Other Notes
Crompton, M., et al. 1999. Biochem. J.341, 233.
Yu, W.H., et al. 1995. J. Biol. Chem.270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem.268, 23289.
Yu, W.H., et al. 1995. J. Biol. Chem.270, 13998.
McEnry, M.W., et al. 1993. J. Biol. Chem.268, 23289.
The immunogen peptide is 100% conserved in all three isoforms of VDAC (VDAC1, VDAC2, and VDAC3). Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description liquid, Calbiochem® | description - | description IgG fraction of antiserum | description clone S152B-23, purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form purified immunoglobulin |
| species reactivity human, rat | species reactivity human, mouse, rat | species reactivity sheep, human, guinea pig, dog, bovine, rabbit, horse, mouse, rat | species reactivity mouse, human, rat |
| form liquid | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| clone polyclonal | clone polyclonal | clone polyclonal | clone S152B-23, monoclonal |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
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I have used this antibody but I not only get a band at ~35kDa. I get a stronger band at ~100kDa. I just wanted to know if this is something that is known to happen with this antibody and what this off-target band might be. Many thanks for any help.
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