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PHCC340KIT

Scepter 3.0 Handheld Automated Cell Counter

Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors

Scepter 3.0 Handheld Automated Cell Counter

Synonym(s):

Cell counting instrument


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UNSPSC Code:
41131501
NACRES:
NC.56

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description

Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors

manufacturer/tradename

Scepter

technique(s)

cell counting: suitable

particle size

4-18 μm

compatibility

for use with cells sized 4.0–25.0 μm

shipped in

ambient

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PHCC360KITPHCC360500PHCC340050
compatibility

for use with cells sized 4.0–25.0 μm

compatibility

for use with cells sized 4.0–25.0 μm

compatibility

Scepter for use with cells sized 8.0–25.0 μm

Not compatible with Scepter 2.0 Instruments

compatibility

Scepter for use with cells sized 5.0–15.0 μm

Not compatible with Scepter 2.0 Instruments

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

description

Rapid cell counts in a handheld easy to use format, includes pkg of 40 μm Scepter 3.0 Cell Counter Sensors

description

-

description

-

description

-

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

particle size

4-18 μm

particle size

6-36 μm

particle size

6-36 μm

particle size

4-18 μm

General description

The Scepter 3.0 Handheld Automated Cell Counter delivers rapid cell counts in a handheld, easy to use format. Intended for research use only, Scepter 3.0 has been tested with cell types representative of those in use today. After preparing a dilution of the cell culture of interest, on-screen instructions guide the user through the sampling process. In less than 30 seconds after the Scepter Cell Counter aspirates a sample into the Scepter Cell Counter Sensor, the Scepter cell counter′s screen displays:

  • Cell concentration
  • Average cell size
  • Average cell volume
  • Histogram of size or volume distribution
The device stores up to 999 counts, which can be downloaded to the user′s computer and uploaded for further manipulation.

The Scepter 3.0 Cell Counter is compatible with both the 40uM and 60uM Scepter 3.0 Cell Counter Sensors. This kit (PHCC340KIT) includes a free 50-pack of 40uM sensors.

Other Notes

  • 1 Scepter 3.0 Automated Handheld Cell Counter
  • 1 Test Bead Vial
  • 1 Charging Station
  • 50 Sensors (40μM)
The Scepter 3.0 Cell Counter is not compatible with previous generation sensors; only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products.

Storage Class Code

10-13 - German Storage Class 10 to 13

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  
  1. Will Scepter 3.0 be able to quantify bacterial cultures? How many reads is it possible with a single counter sensor?

    1 answer
    1. No, bacteria are below the size threshold for counting on the Scepter. Sensor tips are designed to be single use.

      Helpful?

  2. Can it count nuclei, specifically isolated from human aortic endothelial cells?

    1 answer
    1. While most mammalian nuclei are around 6 micrometers (um), which is at the low end of detection, this size range can also include other cell debris that is often gated out during analysis. Additionally, since the counts are displayed as a normal distribution, the full profile may not be visible at the low end of detection. Generally, it's not recommended to use this method for small nuclei, but the Scepter may work for some cells with larger nuclei.

      Helpful?

Reviews

Active Filters

  1. Barcelona, Spain
    • Review 1
    • Votes 18
    3 out of 5 stars.

    Disappointing upgrade from Scepter 2. It has no accompanying analysis software.

    As a long term user of the Scepter 2 counter and its accompanying software to monitor the density, size and health of our cells we were pleased to take delivery of a Scepter 3 counter.
    While the Scepter 3 counter itself is simple to use and connectivity is good it is the software we use with the Scepter 2 that makes it really useful to us. The Scepter 3 comes with no such software so if you want to compare/overlay histograms you have to extract them from the optional csv files and do it manually. Similarly there is no way to perform a calculation of percentage gated cells (simple measure of viability) without manual extraction and analysis of the data.
    This may sound a trivial point but when we are continuously monitoring multiple cell lines at different time points post infection/transfection the software is essential.
    Unfortunately the lack of software makes the Scepter 3 no longer an appropriate instrument for our tissue culture analysis requirements.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for taking the time to leave a review. Your feedback is valuable and has been handed over to our Product Management team for further review. Our R&D team will suggest alternative solutions for analyzing data from Scepter 3.0. We will communicate these solutions to customers through local Account Managers.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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