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Peroxidase (POD)

Name: Peroxidase (POD)
Brand: ROCHE

grade I, from horseradish

Synonym(s):

POD, peroxidase

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About This Item

UNSPSC Code:

12352204

EC Number:

1.11.1.7 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

Biological source:

horseradish

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Properties

biological source

horseradish

form

lyophilized (salt-free)

specific activity

≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.), 250 U/mg

packaging

pkg of 25,000 U

manufacturer/tradename

Roche

technique(s)

protein detection: suitable

optimum pH

6.0-6.5

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Donor:hydrogen-peroxide oxidoreductase

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Application

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.
Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.

Analysis Note

Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase
Purity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item	10814407001	11428861001	P1709
Roche 10108090001 Peroxidase (POD)	Roche 10814407001 Peroxidase (POD)	Roche 11428861001 Peroxidase (POD), activated	Sigma-Aldrich P1709 Peroxidase, Maleimide Activated from horseradish
description grade I, from horseradish	description EIA grade, from horseradish	description from horseradish	description lyophilized powder, >200 units/mg protein
specific activity 250 U/mg, ≥250 units/mg protein (At 25 °C with guaiacol and H₂O₂ as the substrates.)	specific activity ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)	specific activity 800 units/mg protein (at 25 °C with ABTS and H₂O₂ as substrate (pH 5.0).)	specific activity >200 units/mg protein
biological source horseradish	biological source horseradish	biological source horseradish	biological source horseradish
form lyophilized (salt-free)	form lyophilized (salt-free)	form lyophilized	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C (−15°C to −25°C)	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in -
optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH 6.0-6.5	optimum pH -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Precautionary Statements

P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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