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Product Name
DNase I, from bovine pancreas
biological source
bovine pancreas
form
lyophilized
packaging
pkg of 100 mg
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
Quality Level
1 of 4
This Item | 10104159001 | D4527 | AMPD1 |
|---|---|---|---|
| biological source bovine pancreas | biological source bovine pancreas | biological source bovine pancreas | biological source - |
| technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable | technique(s) RT-PCR: suitable |
| form lyophilized | form lyophilized | form lyophilized powder | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - |
| Quality Level 100 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
Biochem/physiol Actions
Features and Benefits
- Lyophilized
- Filtered through 0.2 μm pore size membrane before lyophilization
- Glycoprotein and double-strand-specific endonuclease
General description
DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[7] DNase I is one of the most well characterized endonucleases of mammalian origin.[8]
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.
Preparation Note
Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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