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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
A0576
Agarose
Low EEO
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
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Product Name
Agarose, Low EEO
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
type
Type I-B
form
powder
technique(s)
electrophoresis: suitable
impurities
≤7% water
ash
≤0.25%
turbidity
≤4 NTU (1.5% gel)
EEO
≤0.12
mp
86.5-89.5 °C
transition temp
gel point 36 °C ±1.5 °C (1.5% gel)
gel strength
≥1800 g/cm2 (1% gel)
≥3200 g/cm2 (1.5% gel)
anion traces
sulfate (SO42-): ≤0.12%
Quality Level
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This Item | A0169 | A6013 | A4679 |
|---|---|---|---|
| gel strength ≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel) | gel strength ≥1200 g/cm2 (1% gel) | gel strength ≥1200 g/cm2 (1% gel) | gel strength ≥1200 g/cm2 (1% gel) |
| EEO ≤0.12 | EEO 0.09-0.13 | EEO 0.09-0.13 | EEO 0.09-0.13 |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable, immunodiffusion: suitable, immunoelectrophoresis: suitable |
| impurities ≤7% water | impurities ≤7% water | impurities ≤10% water | impurities ≤7% water |
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application
- to form the photosynthetic slab[1]
- in comparative study, to compare it with native agar, and agarose of Gracilaria dura[2]
- to estimate the amino acid concentrations in agarose and agar samples[3]
- as immobilisation substrates for enzyme immobilisation[4]
- for separating high molecular weight nucleic acids at low gel concentrations
Biochem/physiol Actions
General description
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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