This product has not been tested for use in cell culture. Currently, a cell culture tested caffeine product is not available. However, this item has been used successfully in cell culture applications by other researchers. Please refer to the PEER REVIEWED PAPERS section above for various publications. Please see the link below to review one example:
https://pmc.ncbi.nlm.nih.gov/articles/PMC4216694/
C0750
Caffeine
powder, ReagentPlus®
Synonym(s):
Guaranine, Methyltheobromine, 1,3,7-Trimethylxanthine
Sign In to View Organizational & Contract Pricing
Select a Size
Pricing and availability is not currently available.
About This Item
Empirical Formula (Hill Notation):
C8H10N4O2
CAS Number:
Molecular Weight:
194.19
Beilstein:
17705
EC Number:
MDL number:
UNSPSC Code:
12352210
PubChem Substance ID:
NACRES:
NA.21
Skip To
Quality Level
product line
ReagentPlus®
form
powder
mp
234-236.5 °C (lit.)
solubility
H2O: 15 mg/mL
SMILES string
CN1C(=O)N(C)c2ncn(C)c2C1=O
InChI
1S/C8H10N4O2/c1-10-4-9-6-5(10)7(13)12(3)8(14)11(6)2/h4H,1-3H3
InChI key
RYYVLZVUVIJVGH-UHFFFAOYSA-N
Gene Information
human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142), PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577)
mouse ... Adora2b(11541)
rat ... Adora1(29290), Adora2a(25369)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | C7731 | C-051 | C6035 |
|---|---|---|---|
| mp 234-236.5 °C (lit.) | mp 234-236.5 °C (lit.) | mp - | mp - |
| product line ReagentPlus® | product line - | product line - | product line - |
| form powder | form crystalline, powder | form liquid | form - |
| solubility H2O: 15 mg/mL | solubility H2O: soluble 18.7 g/L at 16 °C | solubility - | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142), PRKAR1A(5573), PRKAR1AP(5574), PRKAR1B(5575), PRKAR2A(5576), PRKAR2B(5577) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140), PDE4B(5142) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140) | Gene Information human ... ADORA1(134), ADORA2A(135), ADORA2B(136), ADORA3(140) |
General description
Caffeine is a widely ingested pharmacologically active substance and is known to affect the central nervous system. It belongs to the class of methylxanthines.[1] It is a naturally occurring alkaloid found in coffee, cocoa and other plants. Caffeine might be associated with osteoporosis, cardiovascular risk, arrhythmia and gastrointestinal disturbances.[2] Caffeine is used to treat orthostatic hypotension,[3] bronchopulmonary dysplasia and apnea of prematurity (AOP).[4]
Application
Biochem/physiol Actions
Caffeine is a natural stimulant that is most commonly found in coffee, tea and various other drinks. It is known for its effect on the central nervous system. It is also associated with insomnia, increased heart rate, digestive issues, creating anxiety and jitteriness. Caffeine is also associated with withdrawal symptoms like headaches, fatigue, irritability.
Features and Benefits
High purity product that can be used readily for HPLC applications along with other research applications.
Legal Information
ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Keep tightly sealed.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree
Noirot M, et al.
Annals of Botany, 92(2), 259-264 (2003)
A simple method for immunohistochemical staining of zebrafish brain sections for c-fos protein expression
Chatterjee D, et al.
Zebrafish, 12(6), 414-420 (2015)
Orthostatic hypotension in the elderly: diagnosis and treatment
Gupta V and Lipsitz LA
The American Journal of Medicine, 120(10), 841-847 (2007)
Apnea of prematurity: from cause to treatment
Zhao J, et al.
European Journal of Pediatrics, 170(9), 1097-1105 (2011)
Metabolic effects of spices, teas, and caffeine
Westerterp-Plantenga M, et al.
Physiology & Behavior, 89(1), 85-91 (2006)
-
Is this cell culture grade caffeine?
1 answer-
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How do I make a stock solution of caffeine catalog number C0750?
1 answer-
The stock solutions of caffeine are prepared by dissolving the appropriate amounts of caffeine in distilled water, ethanol, methanol, or chloroform. This product is tested for solubility at 15 mg/mL in water. Please note that the choice of solvent and concentration would depend on the specific application requirements.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service