Asialofetuin from fetal calf serum

Name: Asialofetuin from fetal calf serum
Brand: SIGMA

Type II (Sigma designation)

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.61

MDL number:

MFCD00130561

Form:

powder

Biological source:

bovine

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Properties

biological source

bovine

Quality Segment

200

type

Type II (Sigma designation)

form

powder

technique(s)

activity assay: suitable, inhibition assay: suitable

impurities

salt, essentially free, ≤1.0% N-acetylneuraminic acid

solubility

H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow

storage temp.

2-8°C

Description

Application

Asialofetuin from fetal calf serum is suitable for use:

in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
in the inhibition of zona pellucida hardening of mouse oocytes
as an acceptor in sialyltransferase enzyme activity assay

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Biochem/physiol Actions

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Preparation Note

Enzymatic cleavage using neuraminidase

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This Item	A4781	F2379	F3004
Sigma-Aldrich A1908 Asialofetuin from fetal calf serum	Sigma-Aldrich A4781 Asialofetuin from fetal calf serum	Sigma-Aldrich F2379 Fetuin from fetal bovine serum	Sigma-Aldrich F3004 Fetuin from fetal bovine serum
description Type II (Sigma designation)	description Type I (Sigma designation)	description lyophilized powder, suitable for cell culture	description lyophilized powder
biological source bovine	biological source bovine (calf) serum	biological source bovine serum (fetal)	biological source bovine serum (fetal)
technique(s) activity assay: suitable, inhibition assay: suitable	technique(s) -	technique(s) cell culture \| mammalian: suitable	technique(s) HPLC: suitable, MALDI-MS: suitable
form powder	form powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
solubility H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow	solubility 0.85% sodium chloride: soluble 1 mg/mL	solubility H₂O: 1 mg/mL, colorless to faint yellow or tan	solubility -
impurities salt, essentially free, ≤1.0% N-acetylneuraminic acid	impurities salt, essentially free, ≤0.5% N-acetylneuraminic acid	impurities ≤0.3% free N-acetylneuraminic acid	impurities ~0.2% free N-acetylneuraminic acid