C8180

Cholera Toxin A Subunit from Vibrio cholerae

lyophilized powder

• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00130777
• Form: lyophilized powder

Properties

• form: lyophilized powder
• Quality Segment: 200
• composition: Protein, ~5% Lowry
• availability: not available in Japan
• impurities: ≤0.5% Cholera toxin B subunit (SDS-PAGE)
• storage temp.: 2-8°C

Description

General description

Cholera Toxin (CT) is secreted by Vibrio cholerae. CT upregulates cell surface molecules, such as co-stimulatory molecules and chemokine receptors in antigen presenting cells(APCs), murine and human dendritic cells (DCs). It enhances the secretion of interleukin 1 (IL-1) from macrophages and stimulates their APC function. CT also functions as an immunomodulator.

Application

Cholera Toxin A Subunit from Vibrio cholerae has been used as a specific probe for trimeric G proteins in membrane motility assays.

Biochem/physiol Actions

Catalyzes ADP-ribosylation of the α-subunit of G proteins, reducing GTPase activity and activating the α-subunit; also catalyzes ADP-ribosylation of cell membrane adenylyl cyclase

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide and sodium EDTA

Preparation Note

When reconstituted with 1 mL of water, the solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN₃, and 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.

Safety Information

• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• Pictograms: GHS06
• Signal Word: Danger
• Hazard Codes: H300 + H310,H332,H412
• Precautionary Statements: P262 - P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials