D0672
φX174 DNA Marker Hae III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105335
eCl@ss:
32119000
Form:
liquid
Storage temp.:
−20°C
Pricing and availability is not currently available.
form
liquid
usage
200 uses (0.1 mg size), 40 uses (20 μg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Preparation Note
φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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This Item | |||
|---|---|---|---|
| description for DNA electrophoresis | description for DNA electrophoresis | description for DNA electrophoresis | description solution, pkg of 50 μg (in 200 μl) |
| form liquid | form liquid | form liquid | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability - |
| usage 200 uses (0.1 mg size) | usage 1000 uses (0.5 mg size), 200 uses (0.1 mg size) | usage 40 uses (20 μg size), 200 uses (0.1 mg size) | usage - |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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