D9655
pBR322 Hae III Digest
for DNA electrophoresis
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About This Item
NACRES:
NA.25
UNSPSC Code:
12352202
Pricing and availability is not currently available.
Quality Segment
form
liquid
usage
1000 uses (0.5 mg size), 200 uses (0.1 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Preparation Note
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
Other Notes
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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This Item | |||
|---|---|---|---|
| description for DNA electrophoresis | description for DNA electrophoresis | description for DNA electrophoresis | description for DNA electrophoresis |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level - |
| usage 200 uses (0.1 mg size) | usage 200 uses (0.1 mg size), 40 uses (20 μg size) | usage 40 uses (20 μg size), 200 uses (0.1 mg size) | usage 100 uses (0.1 mg size), 500 uses (0.5 mg size) |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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