The concentration of p-nitrophenylphosphate (pNPP) in this product is 1.5 mg/mL. This item is a ready-to-use product and dilution is not recommended. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/285/284/p7998dat.pdf
P7998
Alkaline Phosphatase Yellow
alkaline phosphatase substrate, chromogenic liquid
Synonym(s):
Alkaline phosphatase substrate solution (pNPP)
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About This Item
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.83
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Product Name
Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution
Quality Level
form
liquid
storage temp.
2-8°C
Related Categories
1 of 4
This Item | N7653 | B1911 | M3168 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.
Application
Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.
Physical form
Ready to use
Preparation Note
Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.
Disclaimer
Stable at least one year at 2-8 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Instructions
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Hi, may I know what is the concentration of this solution? Because I need to do the calibration curve. Thank you
1 answer-
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Can this be used after it has been frozen?
1 answer-
The stability of this solution has not been evaluated at −20°C. The freeze/thaw process may contribute to decomposition of the substrate solution. Signs of degradation include changes in color or turbidity and precipitation of the pNPP.
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