55269-U
HybridSPE®-Phospholipid Ultra solid phase extraction (SPE) Cartridge
cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
Synonym(s):
HybridSPE®-Phospholipid
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About This Item
UNSPSC Code:
41115712
NACRES:
NB.21
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Product Name
HybridSPE®-Phospholipid Ultra, cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
material
PE frit (5-9 μm)
polypropylene hardware
composition
bed wt., 30 mg
packaging
pk of 100
technique(s)
solid phase extraction (SPE): suitable
volume
1 mL
matrix active group
zirconia-based phase
Quality Level
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Related Categories
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This Item | 52794-U | 52798-U | 55261-U |
|---|---|---|---|
| matrix active group zirconia-based phase | matrix active group zirconia-based phase | matrix active group zirconia-based phase | matrix active group zirconia-based phase |
| material PE frit (5-9 μm), polypropylene hardware | material - | material - | material PE frit (20 μm), polypropylene hardware |
| volume 1 mL | volume 0.8 mL | volume 0.8 mL | volume 1 mL |
| composition bed wt., 30 mg | composition bed wt., 15 mg | composition bed wt., 15 mg | composition bed wt., 30 mg |
| packaging pk of 100 | packaging pk of 1 | packaging pk of 20 | packaging pk of 100 |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Features and Benefits
- Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
- Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
- 2-3 step generic procedure
- Minimal to no method development
- Available in 96-well and 1 mL cartridge dimensions
- Ultra cartridge allows the use of "In-cartridge" protein precipitation method
- Filtrate is ultra clean, free of fines, and suitable for direct injection in UHPLC-type applications
General description
HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.
The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.
The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.
Legal Information
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Identification and elimination of ion suppression in the quantitative analysis of sirolimus in human blood by LC/ESI-MS/MS
Mano, N., et al.
Journal of Chromatography. B, Biomedical Applications, 879 (13-14), 968-974 (2011)
Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry
Ismaiel, O., et al.
Journal of Chromatography. B, Biomedical Applications, 878 (31), 3303-3316 (2010)
Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography-tandam mass spectrometry
Hongliang, J,, et al.
Journal of Chromatography. B, Biomedical Applications, 879 (22), 2162-2170 (2011)
Improved sensitivity of sedimentary phospholipid analysis resulting from a novel extract cleanup strategy
Zhu, Z., et al.
Organic Geochemistry, 65, 46-52 (2013)
Pankaj K Kanaujia et al.
Journal of chromatography. A, 1218(38), 6612-6620 (2011-08-25)
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX.
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