Cerebrospinal fluid and plasma distribution of myo-inositol and other polyols in Alzheimer disease.

Cerebrospinal fluid and plasma distribution of myo-inositol and other polyols in Alzheimer disease.

Clinical chemistry (1996-02-01)

H U Shetty, H W Holloway, M B Schapiro

ABSTRACT

Previous studies suggest the presence of increased concentrations of cerebral myo-inositol in Alzheimer disease (AD). To characterize this abnormality further, we quantified myo-inositol and several other polyols in cerebrospinal fluid (CSF) and plasma from 10 AD subjects and 10 healthy age-matched controls by using a gas chromatographic-mass spectrometric technique. The mean CSF concentration and CSF/plasma concentration ratio of myo-inositol in AD were not significantly different from those determined in control subjects. Also, concentration profiles of other polyols were not significantly altered in AD. CSF and plasma myo-inositol concentrations were correlated in control subjects but not in AD subjects. However, a significant correlation between CSF and plasma 1,5-anhydrosorbitol (a polyol internal control) concentrations observed in control subjects was retained in AD subjects.

MATERIALS

Product Number

Brand

Product Description

357421

Sigma-Aldrich

Tungsten, powder, ≤10 μm, ≥99.99% trace metals basis

215414

Sigma-Aldrich

Calcium, turnings, 99% trace metals basis

203904

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

774111

Sigma-Aldrich

Copper, nanopowder, 40-60 nm particle size (SAXS), ≥99.5% trace metals basis

774103

Sigma-Aldrich

Copper, nanopowder, 60-80 nm particle size (APS), ≥99.5% trace metals basis

767484

Sigma-Aldrich

Nickel, sputtering target, diam. × thickness 2.00 in. × 0.25 in., 99.95% trace metals basis

W368401

Sigma-Aldrich

L-Glutamine

PHR1103

Supelco

L-Serine, Pharmaceutical Secondary Standard; Certified Reference Material

PHR1107

Supelco

L-Glutamic acid, Pharmaceutical Secondary Standard; Certified Reference Material

M54058

Sigma-Aldrich

Methylmalonic acid, 99%

W358509

Sigma-Aldrich

L-Phenylalanine, 99%, FCC

266086

Sigma-Aldrich

Copper, powder, <425 μm, 99.5% trace metals basis

215481

Sigma-Aldrich

Zinc, mossy, ≥99%

268496

Sigma-Aldrich

Titanium, powder, −100 mesh, 99.7% trace metals basis

268526

Sigma-Aldrich

Titanium, sponge, 1-20 mm, 99.5% trace metals basis

433667

Sigma-Aldrich

Titanium, evaporation slug, diam. × L 6.3 mm × 6.3 mm, ≥99.99% trace metals basis

343250

Sigma-Aldrich

Silicon, pieces, 99.95% trace metals basis

357634

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

326852

Sigma-Aldrich

Aluminum, foil, thickness 0.25 mm, 99.999% trace metals basis

326860

Sigma-Aldrich

Aluminum, foil, thickness 0.13 mm, ≥99.99% trace metals basis

556130

Sigma-Aldrich

Bismuth, pieces, 1-12 mm, 99.999% trace metals basis

215619

Sigma-Aldrich

Silicon, powder, −325 mesh, 99% trace metals basis

266574

Sigma-Aldrich

Aluminum, foil, thickness 0.45-0.55 mm, 99.999% trace metals basis

338788

Sigma-Aldrich

Aluminum, pellets, 3-12 mm, 99.99% trace metals basis

266957

Sigma-Aldrich

Aluminum, foil, thickness 1.0 mm, 99.999% trace metals basis

349178

Sigma-Aldrich

Copper, foil, thickness 0.25 mm, 99.98% trace metals basis

348864

Sigma-Aldrich

Titanium, wire, diam. 0.5 mm, 99.99% trace metals basis

324930

Sigma-Aldrich

Zinc, powder, <150 μm, 99.995% trace metals basis

398055

Sigma-Aldrich

Succinic acid, ACS reagent, ≥99.0%

267007

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis