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  • Phenolic compounds in grains, sprouts and wheatgrass of hulled and non-hulled wheat species.

Phenolic compounds in grains, sprouts and wheatgrass of hulled and non-hulled wheat species.

Journal of the science of food and agriculture (2014-08-19)
Paolo Benincasa, Angelica Galieni, Anna Chiara Manetta, Roberta Pace, Marcello Guiducci, Michele Pisante, Fabio Stagnari
ABSTRACT

The use of sprouts and young plantlets in human nutrition is increasing because they often contain phytochemicals and other high value nutrients. This is also the case for wheat, although there is no literature for hulled wheat species. Thus we determined total polyphenols, phenolic acids (PAs), fibre and minerals in grains, 5-day-old sprouts and 12-day-old wheatgrass of einkorn (cv. Monlis), emmer (cvs Augeo, Rosso Rubino, Zefiro), spelt (cvs Pietro, Giuseppe), durum wheat (cv. Creso) and soft wheat (cv. Orso). Grains of einkorn and emmer contained twice bound PAs as compared to soft and durum wheat and spelt, with p-coumaric acid accounting for about 50% of total bound PAs. In wheatgrass, differences between species for bound PAs decreased due to a decrease in einkorn and emmer and an increase in soft and durum wheat. In all species, total phenols and free PAs increased passing from grains to sprouts and wheatgrass. Neutral and acid detergent fibre content increased with sprouting only in einkorn and emmer. Our evidence suggests that the grains of einkorn and emmer and the sprouts and wheatgrass of all Triticum species might potentially be valuable for the development of functional foods.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium hydride, in paraffin
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Sodium hydroxide-16O solution, 20 wt. % in H216O, 99.9 atom % 16O
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trans-Ferulic acid, ≥99%
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Methanol solution, suitable for NMR (reference standard), 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
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4-Hydroxybenzoic acid, ReagentPlus®, ≥99%
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Potassium hydride, 30 wt % dispersion in mineral oil
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2-Hydroxycinnamic acid, predominantly trans, 97%
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Potassium, chunks (in mineral oil), 98% trace metals basis
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trans-Ferulic acid, 99%
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Hydrogen chloride, ReagentPlus®, ≥99%
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4-Hydroxybenzoic acid, ReagentPlus®, 99%
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Syringic acid, ≥95% (HPLC)
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5α-Androstan-17β-ol-3-one, ≥97.5%
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Gallic acid, 97.5-102.5% (titration)
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5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
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Acetonitrile, anhydrous, 99.8%
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Nitric acid, ACS reagent, ≥90.0%
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Nitric acid, 70%, purified by redistillation, ≥99.999% trace metals basis
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Caffeic acid, ≥98.0% (HPLC)
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Acetonitrile, electronic grade, 99.999% trace metals basis
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Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)
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Nitric-14N acid solution, ~10 N in H2O, 99.99 atom % 14N
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Acetic acid-12C2, 99.9 atom % 12C
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Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)
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Acetic acid, natural, ≥99.5%, FG
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Acetic acid, ≥99.5%, FCC, FG
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3-Ethyl-2,4-pentanedione, mixture of tautomers, 98%
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Hydrogen peroxide solution, 34.5-36.5%
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Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
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p-Coumaric acid, ≥98.0% (HPLC)