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Endoglycoceramidase II from Rhodococcus sp.

Name: Endoglycoceramidase II from <I>Rhodococcus</I> sp.
Brand: SIGMA

aqueous solution

Synonym(s):

EGCase, ceramide glycanase, glycosyl-N-acetyl-sphingosine 1,1-β-D-glucanohydrolase, oligoglycosylglucosyl(1↔1)ceramide glycohydrolase, oligoglycosylglucosylceramide glycohydrolase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.123 (BRENDA, IUBMB)

MDL number:

MFCD03456712

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

200

conjugate

(Lipid-linked)

form

solution

mol wt

58.9 kDa

shipped in

dry ice

storage temp.

−20°C

Description

Application

Endoglycoceramidase II from Rhodococcus sp. has been used in a study to assess the differentiation of glycosphingolipid-derived glycan structural isomers by liquid chromatography and mass spectrometry. Endoglycoceramidase II from Rhodococcus sp. has also been used in a study to investigate structural and mechanistic analyses of endo-glycoceramidase II.

Physical form

Solution in 20 mM sodium acetate buffer, pH 6.0, containing 0.2% BSA and 0.1% Lubrol PX.

Other Notes

One unit will hydrolyze 1 μmol of asialo-GM1 per min at 37 °C at pH 5.0.

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This Item	L2524	SAE0158	L4025
Sigma-Aldrich E9030 Endoglycoceramidase II from Rhodococcus sp.	Sigma-Aldrich L2524 Lyticase from Arthrobacter luteus	Sigma-Aldrich SAE0158 Chitinase from Streptomyces griseus	Sigma-Aldrich L4025 Lyticase from Arthrobacter luteus
description aqueous solution	description lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret	description chromatographically purified, lyophilized powder, free of DNA contaminants, suitable for Microbiome research	description lyophilized powder, ≥200 units/mg solid
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant -
form solution	form lyophilized powder	form -	form lyophilized powder
shipped in dry ice	shipped in -	shipped in dry ice	shipped in -
mol wt 58.9 kDa	mol wt -	mol wt -	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 300	Quality Level 300	Quality Level 200

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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