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F9291

ANTI-FLAG® BioM2 antibody, Mouse monoclonal

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
biotin conjugate
Clone:
M2, monoclonal
Application:
DB, FLAG purification
Citations:
128
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Quality Segment

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

FLAG purification: suitable, dot blot: suitable (chemiluminescent detection)

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Application

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Learn more product details in our FLAG® application portal.

Physical form

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Preparation Note

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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F3165F1804A9594
ANTI-FLAG® BioM2 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® BioM2 antibody, Mouse monoclonal

ANTI-FLAG® M2 antibody, Mouse monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 antibody, Mouse monoclonal

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Sigma-Aldrich

F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Sigma-Aldrich

A9594

Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

description

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

description

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

description

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

CY3 conjugate

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

technique(s)

FLAG purification: suitable, dot blot: suitable (chemiluminescent detection)

technique(s)

western blot: 10 μg/mL (Protein A), FLAG purification: suitable

technique(s)

FLAG purification: suitable

technique(s)

FLAG purification: suitable, direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000337110
    0000481893
    0000481894
    0000314382
    0000332466

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