05-1000X
Anti-Phosphoserine Antibody, clone 4A4
clone 4A4, Upstate®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
phosphorylation (pSer)
1 of 4
This Item | 05-1000 | 16-455 | AB1607 |
|---|---|---|---|
| species reactivity vertebrates | species reactivity vertebrates | species reactivity - | species reactivity - |
| antibody form purified antibody | antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody |
| clone 4A4, monoclonal | clone 4A4, monoclonal | clone 4A4, monoclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
General description
Dependent upon the molecular weight of the serine phosphorylated protein being detected.
The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
Immunogen
Phosphoserine coupled to KLH
Application
Anti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Biochem/physiol Actions
Serine-phosphorylated proteins from all species
Physical form
50 µL of PBS with 0.05% sodium azide with 30% glycerol.
Format: Purified
Protein G-Sepharose Chromatography
Preparation Note
2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Analysis Note
Routinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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