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About This Item
UNSPSC Code:
41106217
NACRES:
NA.74
Agency
ISO 11290
Quality Level
sterility
sterile
form
plate
feature
ready-to-use
packaging
box of 20 plates
manufacturer/tradename
ReadyPlate™
plate diam.
90 mm
pH
7.2 ( in H2O)
application(s)
environmental
food and beverages
microbiology
water monitoring
storage temp.
2-8°C
suitability
Listeria spp.
General description
ReadyPlate™ Listeria Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of Listeria monocytogenes and other Listeria sp. from food and feeding stuff. This medium contains the chromogenic compound 5-bromo-4- chloro-3-indolyl-β-D glucopyranoside, a substrate for the detection of β-glucosidase. This enzyme is commonly produced by all Listeria sp. that appear on the medium as blue-green colored colonies. Differentiation of Listeria monocytogenes from other Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.
This basic pH of the medium, ensures good growth for a broad range of bacteria. The addition of inhibitors results in the reduction of the growth of the majority of concomitant Gram-positive and Gram-negative pathogens, as well as of yeasts and fungi. Selectivity is obtained by the addition of lithium chloride, nalidixic acid, and cycloheximide.
This basic pH of the medium, ensures good growth for a broad range of bacteria. The addition of inhibitors results in the reduction of the growth of the majority of concomitant Gram-positive and Gram-negative pathogens, as well as of yeasts and fungi. Selectivity is obtained by the addition of lithium chloride, nalidixic acid, and cycloheximide.
Application
Listeria Agar are ready-to-use, sterile 90mm plates for isolation and differentiation of Listeria monocytogenes and other Listeria sp. from food and animal feed, environmental samples in the area of food production, food handlings and other materials.
Legal Information
READYPLATE is a trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
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