The kit is compatible with mouse samples for STAT1, 3, and 5 only. However, it is a nonconfigurable kit, so the full panel would need to be purchased.
48-610MAG
MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay
Synonym(s):
Magnetic Bead STAT Kit, STAT Cell Signaling Kit
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
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species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP® technology)
storage temp.
2-8°C
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This Item | 48-623MAG | 48-621MAG | 48-690MAG |
|---|---|---|---|
| species reactivity human | species reactivity human, mouse | species reactivity mouse, human, rat | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) |
General description
The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
Application
- An overnight (4°C) incubation is recommended for best results.
- This assay requires 25 μL diluted cell lysate per well.
- This kit must be run using Assay Buffer 1 (provided).
- 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Research Category
Signaling|Apoptosis & Cancer
Signaling|Apoptosis & Cancer
The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)
Biochem/physiol Actions
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Packaging
96-well plate
Preparation Note
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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