662105

PYR-41

≥90% (sum of two isomers, HPLC), Ubiquitin E1 Inhibitor, solid

• Synonym(s): Ubiquitin E1 Inhibitor, PYR-41, 4-(4-(5-Nitro-furan-2-ylmethylene)-3,5-dioxo-pyrazolidin-1-yl)-benzoic acid ethyl ester, trihydrate, Ubiquitin-Activating Enzyme E1 Inhibitor, PYR-41
• Empirical Formula (Hill Notation): C₁₇H₁₃N₃O₇ · 3H₂O
• Molecular Weight: 425.35
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: Ubiquitin E1 Inhibitor, PYR-41, The Ubiquitin E1 Inhibitor, PYR-41 controls the biological activity of Ubiquitin E1. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
• Quality Level: 100
• Assay: ≥90% (sum of two isomers, HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: dark purple
• solubility: DMSO: 10 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable pyrazone compound that irreversibly (presumably via covalent modification) inhibits ubiquitin-activating enzyme E1 activity (IC₅₀<10 µM in cell-free E1 ubiquitination reactions), while exhibiting little or no activity against E3, E2, or caspase enzymatic activity. Blocks ubiquitination-dependent protein degradation and other ubiquitination-mediated cellular activities. E1 inactivation by PYR-41 or other means has been shown to result in an overall elevation of sumoylation.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C. At room temperature, compound will undergo ~1% decomposition per day.

Other Notes

Yang, Y., et al. 2007. Cancer Res.67, 9472.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable