Yes, yeasts are eukaryotic organisms. α-Amanitin inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase.
A2263
α-Amanitin
From Amanita phalloides, ≥85% (HPLC), RNA polymerase II and III inhibitor, powder
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About This Item
Empirical Formula (Hill Notation):
C39H54N10O14S
CAS Number:
Molecular Weight:
918.97
UNSPSC Code:
12352202
NACRES:
NA.32
PubChem Substance ID:
EC Number:
245-432-2
MDL number:
Beilstein/REAXYS Number:
1071138
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Product Name
α-Amanitin, from Amanita phalloides, ≥85% (HPLC), powder
InChI key
CIORWBWIBBPXCG-UHFFFAOYSA-N
InChI
1S/C39H54N10O14S/c1-4-16(2)31-36(60)42-11-29(55)43-25-15-64(63)38-21(20-6-5-18(51)7-22(20)46-38)9-23(33(57)41-12-30(56)47-31)44-37(61)32(17(3)27(53)14-50)48-35(59)26-8-19(52)13-49(26)39(62)24(10-28(40)54)45-34(25)58/h5-7,16-17,19,23-27,31-32,46,50-53H,4,8-15H2,1-3H3,(H2,40,54)(H,41,57)(H,42,60)(H,43,55)(H,44,61)(H,45,58)(H,47,56)(H,48,59)
SMILES string
CCC(C)C1NC(=O)CNC(=O)C2Cc3c([nH]c4cc(O)ccc34)S(=O)CC(NC(=O)CNC1=O)C(=O)NC(CC(N)=O)C(=O)N5CC(O)CC5C(=O)NC(C(C)C(O)CO)C(=O)N2
biological source
Amanita phalloides
assay
≥85% (HPLC)
form
powder
mol wt
918.97 g/mol
color
white to light yellow
mp
254-255 °C (lit.)
solubility
H2O: 1.0 mg/mL
ε (extinction coefficient)
13,500 at 310 nm in H2O at 1 M
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | A1304 | D4288 | L8511 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥85% (HPLC) | assay ≥85% (HPLC) | assay ≥85% (HPLC) | assay ≥85% (HPLC) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mol wt 918.97 g/mol | mol wt - | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: 1.0 mg/mL | solubility - | solubility water: 50 mg/mL, clear, colorless to faintly yellow | solubility H2O: 50 mg/mL |
Application
Biochem/physiol Actions
Inhibits eukaryotic RNA polymerase II and III; does not inhibit RNA polymerase I or bacterial RNA polymerase; inhibits mammalian protein synthesis.
The major toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but does not inhibit RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.
Disclaimer
When stored frozen in the dark, retained samples were found to be greater than 99% by HPLC after two years.
Preparation Note
Store a 1 mg/ml aqueous stock solution frozen at -20°C. It does not degrade with repeated freeze-thaw cycles. Dilute into the appropriate buffer immediately prior to use. Product is destroyed by concentrated acid or base.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Oral - STOT RE 2
target_organs
Kidney,Blood,Liver,Gastrointestinal tract,Respiratory Tract,Central nervous system
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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Alpha-amanitin poisoning, nephrotoxicity and oxidative stress: an experimental mouse model
Ergin M, et al.
Iranian Red Crescent Medical Journal, 17(8), e28068-e28068 (2015)
Prerna Sethi et al.
Neuroscience letters, 459(2), 100-104 (2009-05-02)
Micro-RNA (miRNA) mediated regulation of messenger RNA (mRNA) complexity in the central nervous system (CNS) is emerging as a critical factor in the control of CNS-specific gene expression during development, plasticity, aging and disease. In these studies, miRNA array and
Whole-genome expression analysis of mammalian-wide interspersed repeat elements in human cell lines.
Davide Carnevali et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 24(1), 59-69 (2016-12-29)
With more than 500,000 copies, mammalian-wide interspersed repeats (MIRs), a sub-group of SINEs, represent ∼2.5% of the human genome and one of the most numerous family of potential targets for the RNA polymerase (Pol) III transcription machinery. Since MIR elements
Evaluation of the genotoxicity of alpha-amanitin in mice bone marrow cells
Marciniak B, et al.
Toxicon, 137, 1-6 (2017)
Emmanuelle Querido et al.
Journal of cell science, 124(Pt 10), 1703-1714 (2011-04-23)
Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we
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Can we use this product (alpha amanitin: A2263) to block RNA PII for yeast cell (S. cerevisiae)?
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