Thymidine is a component of Nutrient Mixture F-12 at 0.73mg/L. This media has an expiration date of 18 months from date of formulation. You will need to determine if this is appropriate for your application.
T1895
Thymidine
powder, BioReagent, suitable for cell culture
Synonym(s):
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil, 1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine deoxyriboside, dT
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About This Item
Empirical Formula (Hill Notation):
C10H14N2O5
CAS Number:
Molecular Weight:
242.23
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-070-4
Beilstein/REAXYS Number:
89285
MDL number:
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Product Name
Thymidine, powder, BioReagent, suitable for cell culture
InChI key
IQFYYKKMVGJFEH-XLPZGREQSA-N
InChI
1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
SMILES string
CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
biological source
synthetic (organic)
product line
BioReagent
assay
≥99%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
186-188 °C (lit.)
solubility
water: 50 mg/mL, clear, colorless
Quality Level
Gene Information
human ... DTYMK(1841)
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Related Categories
1 of 4
This Item | T9250 | 6060 | 1724543 |
|---|---|---|---|
| biological source synthetic (organic) | biological source synthetic (organic) | biological source - | biological source - |
| Quality Level 300 | Quality Level 300 | Quality Level 100 | Quality Level - |
| form powder | form powder | form solid | form - |
| assay ≥99% | assay ≥99% | assay ≥98% (HPLC) | assay - |
| solubility water: 50 mg/mL, clear, colorless | solubility water: 50 mg/mL, clear, colorless | solubility water: 10 mg/mL | solubility - |
| mp 186-188 °C (lit.) | mp 186-188 °C (lit.) | mp - | mp 186-188 °C (lit.) |
General description
Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.[1]
Application
Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:
- as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts[2]
- as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies[3]
- to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells[4]
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Find documentation for the products that you have recently purchased in the Document Library.
Optimization in Drug Discovery: In Vitro Methods (2016)
Human Biochemistry and Disease, 571-571 (2008)
Molecular Biology, Principles of Genome Function: Biology, Molecular biology (2016)
Dominique Desbouis et al.
Journal of medicinal chemistry, 51(21), 6689-6698 (2008-10-08)
Human cytosolic thymidine kinase (hTK1) has proven to be a suitable target for noninvasive imaging of cancer cell proliferation using radiolabeled substrates such as [ (18)F]fluorothymidine ([ (18)F]FLT). However, a thymidine tracer useful for single photon emission tomography (SPECT) based
Serum neurogranin measurement as a biomarker of acute traumatic brain injury
Yang J, et al.
Clinical Biochemistry, 43(13-14), 843-848 (2015)
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How long can I store media supplemented with Product T1895, Thymidine?
1 answer-
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How can I solubilize Product T1895, Thymidine?
1 answer-
This product is soluble in water (50 mg/mL), yielding a clear, colorless solution with heating. For aqueous concentrations higher than 50 mg/mL, solubility can be enhanced with the addition of a small amount of NaOH solution. Add the base drop wise with slow stirring. Thymidine is also soluble in methanol, hot alcohol, hot acetone, hot ethyl acetate, pyridine, glacial acetic acid, and is sparingly soluble in hot chloroform.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What concentration of Product T1895, Thymidine, is used for testing this product for cell culture applications?
1 answer-
The tested working concentration for cell culture for Thymidine is 8 mg/L.
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How can I sterilize a solution of Product T1895, Thymidine?
1 answer-
Solutions can be sterile filtered using 0.2 μm filters (low protein binding). Do not autoclave solutions.
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