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CLLS1069

Sigma-Aldrich

MCF10A CELLS APC (-/-)

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eCl@ss:
32190102

biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

Quality Level

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... APC(324)

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Sigma-Aldrich

CLLS1069

MCF10A CELLS APC (-/-)

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CLLS1211

MCF10A CELLS PVRL4 (-/-)

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CLLS1046

MCF10A CELLS PTEN (-/-)

Sigma-Aldrich

CLLS1042

MCF10A CELLS CDH1 (-/-)

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

OMIM accession no.

611731

OMIM accession no.

609607

OMIM accession no.

601728

OMIM accession no.

602118

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... APC(324)

Gene Information

human ... PVRL4(81607), nectin-4(81607)

Gene Information

human ... PTEN(5728)

Gene Information

human ... CDH1(1105)

General description

MCF10A CELLS APC (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.
Adenomatous polyposis coli (APC) is a tumor suppressor gene. It is located on human chromosome 5q21.

Application

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Biochem/physiol Actions

Adenomatous polyposis coli (APC) plays an important role in cell division, adhesion and migration. Mutations in APC gene is associated with colorectal carcinomas.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 5q21

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

Product No.
Description
SDS

  • MCF10A CONTROL CELLS 1 vial

  • MCF10A CELLS APC (-/-) 1 vial


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Epigenetic regulation of APC in the molecular pathogenesis of gallbladder cancer.
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The Indian Journal of Medical Research, 143(S1), S82?S90-S82?S90 (2016)
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Li Y, et al.
Oncogene, 35(35), 4558?4568-4558?4568 (2016)

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